Frequently asked Questions


Q. What are AQUAOX solutions?

A. AQUAOX solutions are produced by combining two simple ingredients, purified water and Sodium Chloride (NaCl) that are processed by two Electrochemical Activation generators to produce three types of solutions.

AQUAOX Cleaning Solutions (AX-112, AX-122):

Reducing Water (cleaner) is a non-toxic, non-hazardous, chlorine-free cleaning solution that does not produce fumes or contain hazardous “butyl” solvents or other potentially dangerous substances such as phosphates or ammonia. It removes grease, dirt and oil deposits from virtually all surfaces, cleans without scrubbing and does not leave a residue, such as that with chemicals.

AQUAOX Disinfecting Solutions (AX-275, AX-525):

Oxidizing Water (disinfectant/sanitizer) is produced at a near neutral pH where the predominant antimicrobial agent is Hypochlorous Acid (HOCl), an efficient and efficacious species of chlorine that kills bacteria, fungi, molds, viruses, and spores. It is particularly effective because it can achieve a degree of disinfection that cannot be achieved with traditional chemicals while being biodegradable, non-toxic, non-corrosive and absent of residue.

AQUAOXWater Treatment solutions (AX-5000)

Mixed Oxidant Water contains a mixture of chlorine-oxygen compounds which kill harmful pathogens in water at a very fast rate. Users dilute the HOCL/NaOCL solution with water to get the desired ppm for a specific task or dose it into a water distribution system for disinfecting and removal of biofilm.

Q. How does HOCl kill? … compare with bleach?

A. Nature gives a negative charge to the cell wall of a pathogenic microorganism. The negative charge of the pathogenic microorganism’s cell wall repulses the negative charge of the hypochlorite ions (OCL-) in bleach making it a weak disinfectant.

The AQUAOX neutral Hypochlorous acid (HOCl) molecule easily penetrates the cell wall, making it a very effective disinfectant. HOCl effectively penetrates slime layers, cell walls and protective layers of microorganisms.  Microorganisms quickly die or are made non-functional by reproductive failure.  HOCl is 80-100 times more effective and exceeds the disinfecting properties of bleach by up to 300 times.

Q. Is HOCl safe for humans, animals and environment?

A. Yes,  HOCl is non-toxic, non-corrosive, biodegradable, environmentally friendly and absent of residue.

Q. Is HOCL effective against Covid-19?

A. Yes, CDC recommends the use of >200ppm free available chlorine.

Q. What (other) viruses are killed by exposure to HOCL?

A. There are multiple independent laboratory data that confirm efficacy against enveloped and non-enveloped viruses E.g. Aquaox tested its HOCL solutions at a 225ppm strength and these tests confirm 99.9999% efficacy in 30 seconds against e.g.: Coronavirus, Adenovirus, Hepatitus virus, bovine viral virus, polio virus, herpes virus and HIV.

Q. What is kill-time of HOCL against viruses?

A. Most viruses are fully destroyed within 5 to 60 seconds contact time under clean conditions.

Q. Is HOCL is effective against bacteria?

A. Yes, there are multiple independent laboratory data that confirm efficacy against all kinds of bacteria. (a really long list)

Q.What bacteria are killed by exposure to HOCL?

A. Independent test data confirm that HOCL is effective against gram-negative, gram-positive as well aerobic and anaerobic bacteria.

Q. What is kill-time of HOCL against bacteria?

A. Most bacteria are fully destroyed within 5 to 60 seconds contact time under clean conditions.

Q. Is HOCL effective against fungi?

A. Yes, there are multiple independent laboratory data that confirm efficacy against . (another long list)

Q. What fungi is killed by HOCL exposure?

A. Most fungi are fully destroyed within 5 to 60 seconds contact time under clean conditions.

Q. Is HOCL is effective against spores?

A. Yes, independent test data confirm that HOCL is effective against e.g. C.Diff and Candida Aurus within 5 to 10 minutes contact time.

 Q. Are HOCL solutions FDA approved?

A. FDA approved several HOCL solutions for topical (skin) usage, for direct food contact as well for sanitizing food contact surfaces. FDA requires HOCL producers to submit a formulation for either a 510k device approval or submit formulation with FDA for obtaining a Food Contact Notice (FCN)

Q. Are Aquaox solutions FDA approved?

No, simply as Aquaox has not applied for FCN. Aquaox has submitted and obtained a 510k device application, but this approval is exclusively licensed to Innovacyn Inc., that markets AX-250 formulation under one or more of its brand names.

Q. Is use of HOCL allowed by USDA?

A. HOCL is permitted for usage in food preparation, direct food contact as well for sanitizing food contact surfaces. USDA provides guidelines for ppm-level not to exceed.

Q. Is use of Aquaox solutions allowed by USDA?

A. USDA permits usage of HOCL, but puts limits on concentration

Q. Is usage of HOCL recommended by CDC?

A.  CDC recommends the use of sodium hypochlorites with >200ppm for disinfecting hard (non-porous) services as well aerosols.

Q. Is HOCL approved by EPA?

A. EPA does not approve nor endorse any disinfectant or active ingredient. EPA certifies efficacy of pesticides (disinfectants, sanitizers) for use on hard (non-porous) surfaces.

Q. Are AQUAOX solutions EPA certified?

A. AQUAOX has (2) anti-microbial solutions that are registered with the EPA as hospital grade, broad spectrum hard surface disinfectants. AX Disinfectant 275 (AX-275) and AX Disinfectant 525 (AX-525)

Q. What does it mean that AX-275 and AX-525 are both EPA certified Hospital-Grade Broad-Spectrum disinfectants?

A. This means that efficacy of Aquaox disinfectants against target organisms have been verified by EPA.

Q. What is the active ingredient(s) in AX-275 or AX-525?

A. The only active ingredient in AX-275 and AX-525 is Hypochlorous Acid (HOCL) generated by electrolysis of a dilute sodium chloride solution.

Q. What is the difference between AX-275 and AX-525?

A. AX-275 contains 248 to 302ppm HOCL and AX-525 contains 477-577ppm HOCL, measured as Free Available Chlorine (FAC).

Q. What is the pH of AX-275 or AX-525?

A. The pH of both AX-275 and Ax-525 is near neutral; between. 6.3 to 7.3

Q. How many parts per million Free Available Chlorine is in AX-275 or AX-525?

A. AX-275 contains 275 ± 10% ppm HOCL. AX-525 contains 525 ± 10% ppm HOCL.

Q. When do I us AX-275 and when do I use AX-525?

A. We recommend usage of AX-525 in the presence of blood or bodily liquids. We recommend AX-525 on surfaces that have not been pre-cleaned and thus contain a high level on contaminants (organic matter).

Q. How is AX-275 or AX-525 supplied?

A. Both AX-275 or AX-525 solutions can be supplied in: 2,3.5,4,8,16,24 or 32oz bottles. Both AX-275 or AX-525 solutions can be supplied in 1, 5, 15,30,55,275 or 330 gallon containers.

Q. What is the shelf-life of AX-275 or AX-525?

A. The shelflife is not absolute. The shelflife of AX-275 and AX-525 is determined by the lower concentration level as set by the EPA. Thus, as long as AX-275 contains >248ppm FAC, AX-275 is effective as a Hospital Grade Broad Spectrum disinfectant. As long as the AX-525 contain >477ppm FAC, AX-525 is effective as a Hospital Grade Broad Spectrum disinfectant. AX-275 and AX-525 shelflife is minimal 30 days from production.

Q. What do I use to measure PPM of AX-275 or AX-525?

A. Use 0-300ppm chlorine test strips for AX-275. Use 0-1000ppm chlorine test strips for AX-525.

Q. Where do I purchase these Chlorine strips?

A. Purchase with chlorine test strip manufacturer or purchase from Aquaox website

Q. How do I apply AX-275 or AX-525 on surfaces?

A. Wipe, spray or dip Aquaox Disinfectant on a surface with a cloth, wipe, mop, sponge, sprayer or spray applicator. Allow surfaces to air-dry.

Q. Do I need to dilute AX-275 or AX-525?

A. AX-275 and AX-525 are Ready-To-Use disinfectants. No dilution.

Q. Can I fill and use re-fillable spray bottles with AX-275 or AX-525?

A. It is permitted to spray-bottles, sprayers or spray applicators.

Q. For how long are these spray bottles filled with AX-275 or AX-525 effective? What is the shelf-life?

A. Remove AX-275 or AX-525 at or over 1-week-old from the bottle or storage tank. 1 week.

Q. How do I best store AX-275 or AX-525?

A. Store in a closed plastic container in a cool area away from direct sunlight.

Q. Can I spray and let air-dry AX-275 and AX-525?

A. Spray cleaned surfaces and let air-dry. No wiping needed.

Q. Do I need an electrostatic sprayer to disinfect surfaces?

A. No, not at all. An electrostatic sprayer is more efficiently in covering surfaces, but has no effect on efficacy.

Q. How long do I spray surfaces? How frequent?

A. Spray at 1.4-4ft distance from target area. Spray daily or weekly as needed.

Q. How many square feet surface does 1-gallon AX-275 or AX-525 disinfect?

A. Please, refer to instructions of (electrostatic) sprayer, as output of sprayers may vary between 3-9oz/minute.

Q. How long does the surface need to be wet?

A. Sprayed surfaces must remain wet for 10 minutes.

Q. What PPE is required when spraying AX-275 or AX-525?

A. Only precautionary statement is to avoid contact with eyes, so wear safety glasses. Wash hands with soap and water after handling and before eating.

Q. How long one has to wait before it is safe to enter the (sprayed) area?

A. Area can be entered immediately.

Q. Can I spray AX-275 or AX-525 of fabrics, such as upholstery and curtains?

A. Yes, HOCL will not harm surfaces. When applying to a large non-porous surface, allow an overlap of 50% when spraying from top to bottom using a 3ft side-by-side motion.

Q. Can I spray AX-275 or AX-525 on toys, instruments, tools and equipment?

A. Yes, HOCL will not harm surfaces. Will not harm titanium coated, medical grade stainless steel. AX-275 and Ax-525 can be used to decontaminate critical devices prior to sterilization.

Q. Can I spray AX-275 or AX-525 on all materials or surfaces? Are there materials to be avoided?

A. HOCL can be used on all materials. Avoid Brass, Copper, Silver and Gold.

Q. Is AX-275 or AX-525 corrosive?

A. No, unless it reacts with quaternary ammonium, so do not use quaternary ammonium cleaners to pre-clean surfaces.

Q. Can I use AX-275 or AX-525 in a fogger or humidifier?

A Yes, refer to instructions of fogger or humidifier

Q. Is AX-275 or AX-525 toxic or cytotoxic?

A. No, Independent laboratory data confirms biocompatibility of HOCL

Q. Does AX-275 or AX-525 cause allergic and/or asthmatic reactions?

A. No, independent laboratory data confirm biocompatibility of HOCL

Q. The label says AX-275 or AX-525 is a cleaner and disinfectant in one. Does this mean that surfaces do not need to be pre-cleaned?

A. No, this means that efficacy is warranted under dirty circumstances, so that the solution can overcome the bioload (contaminants) on the surfaces when surface remain wet for 10 minutes.

Q. Can I use any cleaner to preclean surfaces?

A. No, do not use quaternary ammonium cleaners and hydrogen peroxide.

Q. Can I use (Clorox, Lysol, Cavi) wipes to pre-clean surfaces?

A. Yes, as these wipes are all bleach wipes, which active ingredient is free available chlorine.

Q. What cleaner is recommended to wipe surfaces clean prior to applying AX-275 or AX-525 disinfectant?

A. AX-112 is recommended to pre-clean all surfaces. A 1:10-1:20 dilution of AX-122 is also recommended.

Q. What is difference between AX-112 and AX-122 cleaner?

A. AX-112 contains ~170ppm Sodium Hydroxide (NaOH). AX-122 contains ~3400ppm Sodium Hydroxide (NaOH). AX-122 is 20 time more concentrated that Ax-112.

Q. What is shelf life of AX-112 or AX-122?

A. At least 2 years from production date.

Q. Can I use AX-112 or AX-122 on any surface?

A. AX-112 can be used on any surface. AX-122 MUST be diluted 1:10-1:20 to be used on any surface

Q. What is the active ingredient(s) of AX-112 and AX-122?

A. Sodium Hydroxide (NaOH) generated from electrolyses of a sodium chloride solution.

Q. What is the pH of AX-112 or AX-122?

A. PH of Ax-112 is 11-12. PH of AX-122 is 12-13

Q. How do I apply AX-112 or AX-122 on surfaces?

A. Wipe, spray, immerse on surfaces with cloth, mop, wipe, sponge or sprayer.

Q. Do I need to dilute AX-112 or AX-122?

A. AX-112 is a ready-to-use multipurpose cleaner; no dilution needed. AX-122 MUST be diluted 1:10-1:20

Q. Can I fill and use re-fillable spray bottles with AX-112 or AX-122?

A. Yes, permitted.

Q. For how long are these spray bottles filled with AX-112 or AX-122 effective? What is the shelf-life?

A. For at least a year.

Q. How do I best store AX-122 or AX-122?

A. Store in a closed plastic container in a cool area away from direct sunlight.

Q. Can I spray and let air-dry AX-112 and AX-122?

A. Yes, but this make no sense as both cleaners emulsify dirt and debris that then need to wiped off the surface using a greenspeed microfiber cloth or mop.

Q. What PPE is required when using applying AX-112 or AX-122?

A. Only precautionary statement is to avoid contact with eyes, so wear safety glasses. Wash hands with soap and water after handling and before eating.

Q. Do I need to apply AX-112 or AX-122 on microfiber cloth or can I use paper, cotton towels or wipes?

A. You can use paper, cotton towels or wipes. However, the absorbency and adherence of a microfiber cloth is superior. Thus removing dirt and debris far more effectively that paper or cotton towels or even wipes that ‘smear’ the surface.

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